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| Registros recuperados: 225 | |
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| Launey, Sophie. |
| The flat oyster Ostrea edulis L. is the indigenous oyster of the Atlantic as well as the Mediterranean coasts of Europe. Its commercial exploitation dates back to Antiquity but its breeding is now threatened by two parasitic protozoa, among which Bonamia ostreae. Various aspects of the genetics of this species' natural and farmed populations have been studied with the aid of microsatellite markers. At first, the implementation and the screening of two partial genomic libraries made it possible to identify 28 new microsatellite loci. Analysis of the segregation of 12 of these loci and of two enzymatic loci shows that most microsatellite loci are transmitted in a Mendelian way, but some loci have significant segregation distortions. Moreover, seven linkage... |
| Tipo: Text |
Palavras-chave: Microsatellites markers; Parasite; Bonamia ostreae; Oyster; Ostrea edulis; Marqueurs microsatellites; Parasite; Bonamia ostreae; Huître; Ostrea edulis. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/these-1919.pdf |
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| Kerr, R.; Ward, G. M.; Stentiford, G. D.; Alfjorden, A.; Mortensen, S.; Bignell, J. P.; Feist, S. W.; Villalba, A.; Carballal, M. J.; Cao, A.; Arzul, Isabelle; Ryder, D.; Bass, D.. |
| Marteilia refringens causes marteiliosis in oysters, mussels and other bivalve molluscs. This parasite previously comprised two species, M. refringens and Marteilia maurini, which were synonymized in 2007 and subsequently referred to as M. refringens ‘O-type’ and ‘M-type’. O-type has caused mass mortalities of the flat oyster Ostrea edulis. We used high throughput sequencing and histology to intensively screen flat oysters and mussels (Mytilus edulis) from the UK, Sweden and Norway for infection by both types and to generate multi-gene datasets to clarify their genetic distinctiveness. Mussels from the UK, Norway and Sweden were more frequently polymerase chain reaction (PCR)-positive for M-type (75/849) than oysters (11/542). We did not detect O-type in... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Marteilia pararefringens; ITS1 rDNA; IGS rDNA; Paramyxida; Ascetosporea; Mytilus edulis; Ostrea edulis. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00444/55566/57177.pdf |
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| Martin, Anne-genevieve; Durand, Jean-louis; Carbonnier, N.; Le Bec, Claude; Langlade, Aime; Claude, Serge. |
| A new process of collecting fIat oyster spat in deep water, based on the use of suspended mussel shell, was perfected between 1978 and 1982. The technique has contributed the continued breeding of the species in Quiberon bay (Brittany ) , in spite of the presence of Bonamia ostreae. In order to quantify the number of spat recruited by this technique each year, a method of estimation was tested during the summer 1985. This paper describes the sampling strategy and the results. |
| Tipo: Text |
Palavras-chave: Ostréiculture; Ostrea edulis; Captage; Naissain; Huître plate. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/00019/13018/9994.pdf |
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| Bachere, Evelyne; Grizel, Henri. |
| A new Haplosporidie has been observed in the flat oyster Ostrea edulis from the Mediterranean Sea. Different development stages have been described in light and electron microscopy. The characteristics of the spores and especially the presence of at least four sporal filaments enable us to distinguish this Haplosporidie from those already described in the Atlantic flat oysters. Different haplosporidies have been described in the oyster. Some are known as being pathogenic, Minchinia costalis (Andrews et al., 1962 and 1978; Couch 1967) and Minchinia nelsoni (Haskin et al., 1966; Farley 1968 and 1975), others, identified only recently, are considered as occasional parasites (Van Banning, 1977). During the research conducted on Bonamia ostreae (Pichot et al.,... |
| Tipo: Text |
Palavras-chave: Pathologie; Haplosporidium; Parasite; Ostrea edulis; Huître plate. |
| Ano: 1982 |
URL: http://archimer.ifremer.fr/doc/1982/publication-6576.PDF |
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| Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois. |
| Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes. |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf |
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| Mourton, Chantal. |
| Oyster culture is, at a world scale, hit by variious epizooties, which are maintly caused by protozoans of the Phylum Ascetospora. Among them, Bonamia ostreae consitutes an appreciated model in order to study, at the cell scale, host-parasite relationships, as well as the immune role of hemocytes. Indeed, this parasite, which reproduces in hemocytes of Ostrea edulis, but cannot develop in Crassostea gigas. This development of an in vitro model for hemocyte-Bonamia relationships is presented. This includes the development and implementation of various techniques : making primocultures, protocol for infection with purified parasites, histological and immunoenzymatic analyses of infection. Such model allowed to study the interaction between host and... |
| Tipo: Text |
Palavras-chave: Modèle; Histologie; Méthodologie; Primoculture; Ostrea edulis; Parasite; Hémocytes; Bonamia ostreae; Ascetospora; Protozoaire; Epizootie. |
| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/1987/rapport-3234.pdf |
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| Robert, Maeva; Garcia, Celine; Chollet, Bruno; Lopez-flores, Inmaculada; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Arzul, Isabelle. |
| Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of <1% and a minimum detection limit of 50 gene copies per reaction. Using... |
| Tipo: Text |
Palavras-chave: Heart imprints; Ostrea edulis; Quantification; Detection; Real time PCR; Bonamia ostreae. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6934.pdf |
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| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan. |
| Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf |
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| Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis. |
| The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These... |
| Tipo: Text |
Palavras-chave: Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2756.pdf |
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| Audemard, Corinne; Le Roux, Frédérique; Barnaud, Antoine; Collins, Catherine; Sautour, B; Sauriau, Pierre-guy; De Montaudouin, X; Coustau, C; Combes, C; Berthe, Franck. |
| Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of... |
| Tipo: Text |
Palavras-chave: Experimental transmission; In situ hybridization; PCR; Paracartia grani; Ostrea edulis; Marteilia refringens. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2778.pdf |
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| Morga, Benjamin; Renault, Tristan; Faury, Nicole; Arzul, Isabelle. |
| Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality in flat oyster stocks in Europe. Control of the disease currently relies on disease management practices and transfer restriction. Previously, massal selections based on survival to challenge to infection with B. ostreae have been applied to produce flat oyster families with resistant progeny. In an attempt to understand the molecular mechanisms involved in disease resistance, differentially expressed sequence tags between resistant and wild Ostrea edulis haemocytes, both infected with the parasite, were identified using suppression subtractive hybridisation. Expression of seven ESTs has been studied using quantitative reverse-transcriptase PCR. The base-line... |
| Tipo: Text |
Palavras-chave: Protozoan; Ostrea edulis; Flat oyster; Transcriptomic; Gene expression. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00071/18218/15790.pdf |
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| Pogoda, Bernadette; Boudry, Pierre; Bromley, Cass; Cameron, Tom C.; Colsoul, Bérenger; Donnan, David; Hancock, Boze; Hugh‐jones, Tristan; Preston, Joanne; Sanderson, William G.; Sas, Hein; Brown, Janet; Bonacic, Kruno; Nordheim, Henning; Zu Ermgassen, Philine S.e.. |
| The Native Oyster Restoration Alliance (NORA) supports the protection and ecological restoration of the native European oyster, Ostrea edulis, and its habitat across its current and historical biogeographical range. NORA works to overcome barriers to the conservation, restoration, and recovery of the European oyster by providing a platform for the NORA community to collaborate and participate in knowledge exchange. NORA seeks to support responsible restoration practice, in compliance with biosecurity and sustainability. Against this background, the NORA community formulated a series of specific recommendations, the Berlin Oyster Recommendation, to support native oyster restoration by developing and applying best practice with the aim to recover healthy and... |
| Tipo: Text |
Palavras-chave: Ecological restoration; Native Oyster Restoration Alliance; Ostrea edulis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00659/77135/78451.pdf |
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| Marteil, Louis. |
| Le Morbihan est le principal centre européen de production et d'élevage de l'huître plate Ostrea edulis L. 3 500 parcs environ sont exploités dans les rivières d'Etel, de Plouharnel, de Crach, St-Philibert, Auray, de Penerf et du Golfe du Morbihan, 1 800 hectares sont ainsi livrés à la culture de la seul huître plate, 150 à 400 millions de naissains (et parfois plus) sont récoltés chaque année sur 12 millions de collecteurs, 3 000 tonnes d'huîtres de 2 ans et surtout de 3 ans sont livrées chaque année aux centres d'affinage ou d'élevage de France (Belon, Cancale, Morlaix, Paimpol, Marennes, etc...) et éventuellement de l'étranger (Angleterre, Irlande, Hollande), 500 tonnes d'huîtres sont livrées directement à la consommation (70 000 colis environ). Le... |
| Tipo: Text |
Palavras-chave: Ostréiculture; Huître plate; Ostrea edulis; Gisement naturel; Captage; Pathologie. |
| Ano: 1954 |
URL: http://archimer.ifremer.fr/doc/00003/11465/8025.pdf |
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| Barbier, Pierrick; Meziane, Tarik; Foret, Martin; Tremblay, Rejean; Robert, Rene; Olivier, Frederic. |
| Marine habitat function has been typically investigated in terms of biogeochemical regulation but rarely in terms of population renewal, which is mainly controlled by recruitment dynamics. The recruitment phase is crucial for organisms with a bentho-pelagic life cycle, such as bivalves, and it regulates the population renewal success. This study provides new insight on the role of temperate benthic habitats on bivalve recruitment, as a function of nursery areas. Six dominant benthic habitats of the Chausey archipelago (Normandy, France) were studied. In each habitat, bivalve recruit assemblages were described at the end of two reproductive seasons. Furthermore, Ostrea edulis juveniles were immerged on each habitat during two months to compare growth... |
| Tipo: Text |
Palavras-chave: Benthic habitats; Bivalve recruitment; Nursery function; Ostrea edulis; Fatty acids. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00367/47863/47845.pdf |
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| His, Edouard; Tige, Gilbert; Rabouin, Marie-agnès. |
| D'Avril 1974 à Décembre 1975, un lot d'huîtres plates provenant du Golfe de Morbihan, zone encore non parasitée, a été placé en élevage dans une claire des Hosses située dans le bassin d'Arcahon. L'infestation des huîtres par Marteilia refringens a été suivie sur coupes histologiques et, conjointement des enregistrements de l'activité valvaire ont été effectués à l'aide de deux ostréographes. L'association de ces techniques a permis de mettre en évidence un dérèglement de l'activité valvaire coïncidant avec l'apparition de Marteilia refringens chez l'hôte. Des réactions caractéristiques de types pathologiques ont été enregistrées chez les huîtres gravement atteintes. |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Pathologie; Maladie; Bassin Arcachon. |
| Ano: 1976 |
URL: http://archimer.ifremer.fr/doc/00015/12625/9510.pdf |
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| Registros recuperados: 225 | |
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